Associate Professor and
Associate Dean of Undergraduate Studies
Ph.D. 1995, University of Alabama, Birmingham
Targeting Glioblastoma Cell Invasion Using Zebrafish Models for 4D Quantitative Imaging
Glioblastoma is an extremely deadly form of brain cancer. With currently available treatments, survival from the cancer is very low with 90% dying after 5 years of being diagnosed. One reason for glioblastoma’s deadly nature is its ability to escaping tumor resection by moving throughout the brain and creating new tumors. In the Greenwood lab, our research focuses on glioblastoma cell invasion mechanisms to find ways to slow or stop cancer cell movement. To study this invasion process, we inject fluorescently dyed human glioblastoma cells into the brain of embryonic zebrafish. The zebrafish are translucent at this stage and allow us to peer into brain and investigate glioblastoma cells in action. By altering the expression of proteins in the brain environment, we hope to discover specific proteins that simulate or inhibit glioblastoma cell invasion so that cancer treatments can be more effective and lead to increased life expectancy.
||Single-cell imaging of human glioblastoma cell invasion in a zebrafish brain. Fluorescently dyed human glioblastoma cells 48hrs after transplantation into the brain of an embryonic zebrafish with eGFP expressing blood vessels. A) Brightfield image of zebrafish with location of confocal 3D imaging. B) Glioblastoma cells (red) can be seen invading into brain tissue from primary mass marked with a yellow arrow. White arrows indicate individual cells migrating along blood vessels (green).